Development of a Plasmodium PCR for Monitoring Efficacy Of Antimalaria…
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작성자 Blair 댓글 0건 조회 0회 작성일 25-11-19 02:54필드값 출력
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There have been studies of accelerating numbers of circumstances of malaria among migrants and travelers. Although microscopic examination of blood smears remains the "gold normal" in prognosis, this technique suffers from inadequate sensitivity and requires appreciable experience. To enhance analysis, BloodVitals insights a multiplex real-time PCR was developed. One set of generic primers focusing on a highly conserved area of the 18S rRNA gene of the genus Plasmodium was designed; the primer set was polymorphic sufficient internally to design four species-specific probes for P. falciparum, BloodVitals wearable P. vivax, P. malarie, and P. ovale. Real-time PCR with species-specific probes detected one plasmid copy of P. falciparum, P. vivax, P. malariae, and BloodVitals review P. ovale specifically. The identical sensitivity was achieved for all species with actual-time PCR with the 18S screening probe. Ninety-seven blood samples have been investigated. For sixty six of them (60 patients), microscopy and real-time PCR outcomes have been compared and had a crude settlement of 86% for the detection of plasmodia. Discordant outcomes have been reevaluated with clinical, molecular, and sequencing knowledge to resolve them. All nine discordances between 18S screening PCR and BloodVitals wearable microscopy have been resolved in favor of the molecular technique, BloodVitals wearable as have been eight of nine discordances at the species stage for the species-particular PCR among the 31 samples positive by both methods. The opposite 31 blood samples have been examined to monitor BloodVitals wearable the antimalaria treatment in seven patients. The variety of parasites measured by real-time PCR fell quickly for six out of seven patients in parallel to parasitemia determined microscopically. This suggests a role of quantitative PCR for the monitoring of patients receiving antimalaria therapy.
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